4 Preliminary OTU analysis using Mothur
The preliminary analysis (alpha_beta_diversity rule
) is part of the bioinformatics analysis. It includes:
- Creating reads count for each group.
- Subsampling for downstream analysis.
- Rarefaction.
- Computing Alpha diversity metrics.
- Computing Beta diversity metrics.
- Getting sample distances.
- Constructing sample phylip tree.
- Generating ordination matrices including PCoA and NMDS.
- More as needed…